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Extra resources for Amino Acids in Animal Nutrition
The recently developed method of capillary electrophoresis (CE) in different separating media (CE, capillary zone electrophonesis (CZE), micellar electrokinetic capillary chromatography (MECC)) for the determination of amino acids has also been extensively studied. Here the amino acid ions or derivatives migrate in the electric field through a separating capillary, for which a wide range of techniques have been described. , 1999). HPLC after precolumn derivatization After precolumn derivatization with non-polar, UV-vis- or fluorescence-active reagents on the amino group, all amino acids can be separated by RP-HPLC.
Another fluorescence-active reagent for precolumn derivatization is FMOC. It reacts quickly with all amino acids at room temperature, but more slowly than OPA. The derivatives are stable and analytical solutions can be stored for several days in a refrigerator. In the presence of water 9-fluorenylmethanol develops from the reagent, which is highly fluorescence-active and elutes in the centre of the amino acid chromatogram. For this reason an extraction step with non-polar solvents is required, a disadvantage if this cannot be done automatically in a special autosampler.
I. and Zapico, J. (1994) Rapid analysis of free amino acids in infant foods. Journal of Liquid Chromatography 17, 4019–4030. L. and Aucoin, P. (1990) Analysis of taurine in feline plasma and whole blood by liquid chromatography with fluorometric detection and confirmation by thermospray mass spectrometry. Journal of Chromatography 526, 375–382. 32 J. C. R. (1999) HPLC method for analysis of free amino acids in fish using o-phthaldialdehyde precolumn derivatization. Journal of Agricultural and Food Chemistry 47, 5100–5107.